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recombinant mouse csf2  (R&D Systems)


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    R&D Systems recombinant mouse csf2
    (A) Bone marrow-derived macrophages (BMM) from WT and Fireko mice were generated by culture in <t>CSF2</t> for 7 days followed by staining and flow cytometry analysis. F4/80/CD11b + cells were gated based on MHCII expression. The relative proportions of MHCII High and Low subsets and their CSF1R MFI are shown. Data show mean and standard deviation, 2-way ANOVA with Sidak’s multiple comparison test. (B) CSF2-derived BMM were incubated with pH Rodo E.coli bioparticles (BP) for 1 h followed by surface staining and analysis by flow cytometry for BP uptake (%) and median fluorescence intensity (MFI) in cell subsets. Data show mean and standard deviation. (C) Representative images of bone marrow from WT or Fireko mice cultured in CSF1, CSF2 or both for 3 days prior to the addition of RANKL and continued culture until day 7. Cells were stained for tartrate-resistant acid phosphatase (TRAP, purple)
    Recombinant Mouse Csf2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 329 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant mouse csf2/product/R&D Systems
    Average 96 stars, based on 329 article reviews
    recombinant mouse csf2 - by Bioz Stars, 2026-04
    96/100 stars

    Images

    1) Product Images from "Depletion and replacement of tissue resident macrophages in mice with germ-line deletion of a conserved enhancer in the Csf1r locus"

    Article Title: Depletion and replacement of tissue resident macrophages in mice with germ-line deletion of a conserved enhancer in the Csf1r locus

    Journal: bioRxiv

    doi: 10.64898/2026.03.22.713539

    (A) Bone marrow-derived macrophages (BMM) from WT and Fireko mice were generated by culture in CSF2 for 7 days followed by staining and flow cytometry analysis. F4/80/CD11b + cells were gated based on MHCII expression. The relative proportions of MHCII High and Low subsets and their CSF1R MFI are shown. Data show mean and standard deviation, 2-way ANOVA with Sidak’s multiple comparison test. (B) CSF2-derived BMM were incubated with pH Rodo E.coli bioparticles (BP) for 1 h followed by surface staining and analysis by flow cytometry for BP uptake (%) and median fluorescence intensity (MFI) in cell subsets. Data show mean and standard deviation. (C) Representative images of bone marrow from WT or Fireko mice cultured in CSF1, CSF2 or both for 3 days prior to the addition of RANKL and continued culture until day 7. Cells were stained for tartrate-resistant acid phosphatase (TRAP, purple)
    Figure Legend Snippet: (A) Bone marrow-derived macrophages (BMM) from WT and Fireko mice were generated by culture in CSF2 for 7 days followed by staining and flow cytometry analysis. F4/80/CD11b + cells were gated based on MHCII expression. The relative proportions of MHCII High and Low subsets and their CSF1R MFI are shown. Data show mean and standard deviation, 2-way ANOVA with Sidak’s multiple comparison test. (B) CSF2-derived BMM were incubated with pH Rodo E.coli bioparticles (BP) for 1 h followed by surface staining and analysis by flow cytometry for BP uptake (%) and median fluorescence intensity (MFI) in cell subsets. Data show mean and standard deviation. (C) Representative images of bone marrow from WT or Fireko mice cultured in CSF1, CSF2 or both for 3 days prior to the addition of RANKL and continued culture until day 7. Cells were stained for tartrate-resistant acid phosphatase (TRAP, purple)

    Techniques Used: Derivative Assay, Generated, Staining, Flow Cytometry, Expressing, Standard Deviation, Comparison, Incubation, Fluorescence, Cell Culture



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    Image Search Results


    (A) Bone marrow-derived macrophages (BMM) from WT and Fireko mice were generated by culture in CSF2 for 7 days followed by staining and flow cytometry analysis. F4/80/CD11b + cells were gated based on MHCII expression. The relative proportions of MHCII High and Low subsets and their CSF1R MFI are shown. Data show mean and standard deviation, 2-way ANOVA with Sidak’s multiple comparison test. (B) CSF2-derived BMM were incubated with pH Rodo E.coli bioparticles (BP) for 1 h followed by surface staining and analysis by flow cytometry for BP uptake (%) and median fluorescence intensity (MFI) in cell subsets. Data show mean and standard deviation. (C) Representative images of bone marrow from WT or Fireko mice cultured in CSF1, CSF2 or both for 3 days prior to the addition of RANKL and continued culture until day 7. Cells were stained for tartrate-resistant acid phosphatase (TRAP, purple)

    Journal: bioRxiv

    Article Title: Depletion and replacement of tissue resident macrophages in mice with germ-line deletion of a conserved enhancer in the Csf1r locus

    doi: 10.64898/2026.03.22.713539

    Figure Lengend Snippet: (A) Bone marrow-derived macrophages (BMM) from WT and Fireko mice were generated by culture in CSF2 for 7 days followed by staining and flow cytometry analysis. F4/80/CD11b + cells were gated based on MHCII expression. The relative proportions of MHCII High and Low subsets and their CSF1R MFI are shown. Data show mean and standard deviation, 2-way ANOVA with Sidak’s multiple comparison test. (B) CSF2-derived BMM were incubated with pH Rodo E.coli bioparticles (BP) for 1 h followed by surface staining and analysis by flow cytometry for BP uptake (%) and median fluorescence intensity (MFI) in cell subsets. Data show mean and standard deviation. (C) Representative images of bone marrow from WT or Fireko mice cultured in CSF1, CSF2 or both for 3 days prior to the addition of RANKL and continued culture until day 7. Cells were stained for tartrate-resistant acid phosphatase (TRAP, purple)

    Article Snippet: 10 7 BM cells were seeded on 100 mm square bacteriological plastic (Sterilin, Thermo-Fisher, Australia) in 25 ml of complete medium (RPMI + 10% FBS, 25 U/mL penicillin, and 25 μg/mL streptomycin (Gibco, Thermo-Fisher, Australia) and differentiated for 7 days with the addition of recombinant CSF1-Fc (100 ng/ml) or recombinant mouse CSF2 (GM-CSF, R&D Systems, Minneapolis, Mn, USA; 50 ng/ml).

    Techniques: Derivative Assay, Generated, Staining, Flow Cytometry, Expressing, Standard Deviation, Comparison, Incubation, Fluorescence, Cell Culture